Hannes Steinkellner

Biography

Dr. Hannes Steinkellner is currently working as a Research Associate (Postdoc) in the Department of Center for Pathobiochemistry and Genetics (Institute of Medical Genetics ), Medical University of Vienna ,  Austria. His research interests includes Pathobiochemistry and Genetics. He /she is serving as an editorial member and reviewer of several international reputed journals. Dr. Hannes Steinkellner is the member of many international affiliations. He/ She has successfully completed his Administrative responsibilities. He /she has authored of many research articles/books related to Pathobiochemistry and Genetics.

Research Interest

Our main research focus is the development and investigation of TAT (transactivator of transcription)- fusion proteins for neurodevelopmental and neurodegenerative disorders like RETT syndrome, Friedreich's Ataxia and Spinal Muscular Atrophy. The technology requires the synthesis of a fusion protein, linking the TAT transduction domain to the molecule of interest using a bacterial expression vector, followed by the purification of this fusion protein under either soluble or denaturing conditions. The purified fusion protein can be directly added to mammalian cell culture or injected in vivo into mice. Protein transduction occurs in a concentration-dependent manner, achieving maximum intracellular concentrations in less than 5 min, with nearly equal intracellular concentrations between all cells in the transduced population. Full-length TAT fusion proteins have been used to address a number of biological questions, relating to cell cycle progression, apoptosis, and cellular architecture [Dowdy SF et.al., Methods 2001].