Doucet Nicolas
Enzymatic engineering
Institut national de la recherche scientifique
Canada
Biography
Dr. Nicolas Doucet obtained his BS in biochemistry from Université Laval (BS Biochemistry, 2000) and successively completed a master's degree (MS Biochemistry, 2004) and a doctoral thesis in enzyme engineering at the University of Montreal (Ph.D. Biochemistry, 2007 ). After completing a protein NMR postdoctoral fellowship in the Department of Chemistry at Yale University (with J. Patrick Loria, 2007-2010), Dr. Doucet joined the faculty at INRS-Institut Armand-Frappier in 2010.
Research Interest
Understanding the Importance of Residual Motion in the Enzyme Industry, Protein Engineering, Nanotechnology and Drug Design. The aim of this study is to determine the role of the enzyme in biocatalysts. To address this important problem, our molecular biology, enzyme kinetics, and molecular modeling. Using this approach, we focus on achieving the following goals: 1 - To understand how the amino acid sequence and local structural environment of conserved residues determines the motional and functional properties of enzymes. 2 - To investigate how functionally and structurally related enzymes retain their motional properties within similar folds. 3 - To modify, reproduce and apply this 'structure-function' and 'flexibility-function' information to the design of new and improved molecular biocatalysts.
Publications
-
Gagné D, Narayanan C, Nguyen-Thi N, Roux LD, Bernard DN, Brunzelle JS, Couture JF, Agarwal PK, Doucet N. Ligand binding enhances millisecond conformational exchange in xylanase B2 from Streptomyces lividans. Biochemistry. 2016 Jul 21;55(30):4184-96.
-
Agarwal PK, Doucet N, Chennubhotla C, Ramanathan A, Narayanan C. Chapter Twelve-Conformational Sub-states and Populations in Enzyme Catalysis. Methods in enzymology. 2016 Dec 31;578:273-97.
