Dr. Yu Fu
Professor
Institute of Microbiology
Chinese Academy of Sciences
China
Biography
2003-2008: Ph.D. Department of Microbiology and Immunology, University of Saskatchewan, Canada 2000-2003: MSc. Department of Microbiology and Immunology, University of Saskatchewan, Canada 1994-1997: MSc. Institute of Genetics, Chinese Academy of Sciences, China 1990-1994: B.S. Department of Life Sciences, Hubei University, China 2012-present: Professor, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences 2008-2012: Research fellow, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School 1999-2000: Research assistant, Friedrich Miescher Institute, Novartis Research Foundation, Switzerland 1997-1999: Research assistant, Institute of Genetics, Chinese Academy of Sciences, China
Research Interest
Saccharomyces cerevisiae is an ideal eukaryotic model organism in molecular and cell biology. We are using this organism to elucidate the mechanisms which underlie DNA replication, DNA damage repair and aging in eukaryotes. Our laboratory is interested in applying single-molecule, biochemical and genetic methods to study the following areas: Eukaryotic DNA replication: Using single-molecule assays, we are currently exploring the molecular process of DNA replication initiation, elongation and termination in yeast cell-free extracts. We are seeking to understand the functions of DNA replicative helicase in different steps of DNA replication. Dissecting DNA repair pathways in Eukaryotes: We aim to develop microfluidic-based single-molecule approaches to study DNA repair in yeast cell-free extracts. We are most interested in exploring the mechanistic details of replication-coupled DNA repair pathways. Aging in budding yeast: One of the common methods to measure aging in Sacchromyces cerevisiae is Replicative Life Span, which measure the amount of times a yeast cell buds. We are particularly interested in determining the biochemical and epigenetic events in yeast senescence and life span resetting during gametogenesis.
Publications
-
3. Fu Y, Zhu Y, Zhang K, Yeung M, Durocher D and Xiao W (2008) Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp. Cell 133(4): 601-611
-
2. Fu YV and Walter JC (2010) DNA replication: metazoan Sld3 steps forward. Current Biology20(12): 515-517
-
1. Fu YV, Yardimci H, Long D, The Ho V, Guainazzi A, Bermudez V, Hurwitz J, van Oijen A, Schärer O, Walter JC. (2011) Selective bypass of a lagging strand roadblock by the eukaryotic replicative DNA helicase. Cell146(6): 931-941
