Dr. Yogesh Kolekar

Biography

  Experience Executive Officer Company NameSerum Institute of India Limited Dates EmployedAug 2015 – Present   Employment Duration2 yrs 2 mos LocationPune Area, India Working in vaccine production and research Research Associate Company NameNational Chemical Laboratory Dates EmployedApr 2012 – Jul 2015   Employment Duration3 yrs 4 mos LocationPune Area, India Protein drug interaction analysis by using mass spectrometry - Screening and Investigation of Antiglycation molecules  - Label free and gel based proteome quantification gel electrophoresis analysis (1D and 2DE), western blot analysis (1D and 2DE), 2DE image analysis (PDQuest advanced). - Expertise in handling, sample preparation protein digestion (in-solution and in-gel) for instruments including LC ESI Q-TOF MS/MS (Synapt nano LC-MS) and UPLC-HR MS (Thermo Orbitrap systems Q-Exactive) - Proteomics data analysis by using tools- Protein Lynx Global Server (PLGS), Proteome Discoverer (PD 1.4) and KEGGs pathways. - Anti-diabetic drug screening, in vitro identification of AGE inhibitors by standard AGE fluorescence assay and PTMs identification - In vivo studies on (BALB/c mice) studies of the AGE (advance glycation endproducts) or diabetic inhibition studies of drugs and organic molecules. Postdoc fellow Company NameKTH Dates EmployedJun 2010 – Aug 2011   Employment Duration1 yr 3 mos LocationStockholm, Sweden 1. Arsenate reductase assay and identification of genes responsible for arsenic removal, 2. Manipulation of nucleotide sequences, Data analysis by statistical methods, phylogenetic analysis. Submission of nucleotide sequences in Genbank  

Research Interest

 Protein expression and cloning studies, primer designing, PCR optimisation, transformation and purification