Himansh Upadhyaya
Executive Officer
(Recombinant & combination vaccines-QC)
Serum Institute of India
India
Biography
Experience Executive Officer (Recombinant & combination vaccines-QC) Company NameSerum Institute of India Limited Dates EmployedJul 2015 – Present Employment Duration2 yrs 3 mos LocationPune, Maharashtra, India Working in Quality Control (Recombinant Proteins & combination vaccines) for testing and release of Hepatitis B, Pentavalent, DTP-Hib, DTP-HB and other combination vaccines. Development of DTPHB-IPV-Hib vaccine. Associate Research Scientist Company NameZydus Group Dates EmployedMar 2015 – Jun 2015 Employment Duration4 mos LocationAhmedabad Area, India * Incharge of ADL-Virology (Molecular biology, Electrophoresis & Cell biology laboratory) * New method development for viral & recombinant protein vaccines (Hepatitis b, HPV 16L1, 18L1 & Influenza (FLU) vaccines. Scientist III (Vaccines) Company NameU.S. Pharmacopeia Dates EmployedJan 2014 – Feb 2015 Employment Duration1 yr 2 mos LocationHyderabad Area, India Elemental analysis using ICP-OES for calcium in vaccines Circular Dichroism studies of new vaccine candidates. Operation & validations on DIONEX HPAEC-PAD for O-acetyl determination. CZE (Capillary zone electrophoresis) for IEF determination in vaccines. Successful completion of monographs for Meningococcal A & X polysaccharides at USP. Scientist II (Vaccines) Company NameU.S. Pharmacopeia Dates EmployedNov 2012 – Dec 2013 Employment Duration1 yr 2 mos LocationHyderabad, Telangana, India ⦠Elemental analysis using ICP-OES for Aluminum, Phosphorous & Thiomersal (Mercury) in vaccines. ⦠Cesium estimation in Hepatitis B Bulk using ICP-MS. ⦠Operation & validations on DIONEX HPAEC-PAD for Total polysaccharide determination. ⦠Operation & validations on Waters HPLC (RP, SEC) for various purity determinations in vaccines. ⦠ELISA, Dot blot, immunodiffusion for vaccines. ⦠CZE (Capillary zone electrophoresis) for EPO. ⦠CHNS analysis for proteins. ⦠N-terminal automated micro-secquencing for proteins using the Edman degradation reaction. ⦠Physicochemical Analysis of Raw materials, bulk and finished goods.. ⦠Analytical Method validations for various physicochemical, Spectrophotometric tests, etc. ⦠Successful completion of monographs for Hepatitis B, Hib-TT & Tetanus bulks and vaccines, DTP and Pentavalent vaccine at USP. Media (1) This position has 1 media U.S. Pharmacopeial Convention U.S. PharmacopeialConvention This media is a link Senior Scientific Officer at Quality control department (Vaccines) Company NamePanacea Biotec Ltd Dates EmployedOct 2008 – Nov 2012 Employment Duration4 yrs 2 mos LocationMohali, Punjab Technical Skills:- • All the Quality control tools like Change control, Deviations, Out of specification (OOS), Incidents, Risk analysis. • Analytical Method validations for various physicochemical spectrophotometric tests, etc. • Titrations for analysis of various chemicals as per monographs given in Pharmacopoeia. • Protein estimation using lowry method, commassie method. • Protein nitrogen using kjedhl-dhal method. • All raw materials tests. • Packing material tests (hydrolytic resistence) i.e leeching test. • Vaccine tests like formaldehyde estimation, aluminium gel estimation, carbohydrates. • Agarose gel electrophoresis. • Centrifugation. • Thin layer chromatography. • SDS-PAGE. • Spectroscopy (UV and Visible). • Media preparation. • Handling and maintenance of microbial cultures. • Cell culture tests like cell counting, subculturing, passaging, thawing of cell lines like HELA, etc. • Specific optical rotation using polarimeter. • Refractive index using refractometer. • I.R spectrum using solid and liquid samples. • Assays of chemicals using titremetric methods. • Tests like loss on drying, sulphated ash, loss on ignition. • Tests like disintegration, dissolution, friability testing of tablets, etc. • Purity of methanol, Iso propyl alcohol, glycerine using gas chromatography. • Limit tests like heavy metals, chlorides, sulphates, iron, etc. • Other tests used in raw material, packing material, in process vaccines, final arm of vaccine.s
Research Interest
Immunology, vaccines, MAbs