Shubham Patwardhan

Biography

  Experience Assistant Manager Company NameSerum Institute of India Limited Dates EmployedSep 2017 – Present   Employment Duration1 mo LocationPune, Maharashtra, India Executive Officer Company NameSerum Institute of India Limited Dates EmployedMay 2014 – Sep 2017   Employment Duration3 yrs 5 mos LocationPune Area, India Development of Downstream processing steps for Pneumococcal conjugate vaccine. Preparing and review of documents like SOPs, BMRs, Equipment and Qualification documents. Officer Company NameSerum Institute of India Limited Dates EmployedAug 2011 – May 2014   Employment Duration2 yrs 10 mos LocationHadapsar Development of Downstream processing steps for Pneumococcal conjugate vaccine. Process development for Meningiococcal X conjugate vaccine development. Expertise in TFF, Chromatography systems, HPLC Project Trainee Company NameNational Centre For Cell Science Dates EmployedSep 2010 – Aug 2011   Employment Duration1 yr “Global Gene Regulation by P53 and SMAR1” Working on ChIP-Seq data analysis, to find the genes regulated globally by P53 in modulation with SMAR1. The work involves key techniques like Western Blot, Cell Culture, Transfection, Real-Time PCR. Currently learning techniques like AFM, Raman Confocal Microscopy. Project Trainee Company NameNational AIDS Research Institute, Pune Dates EmployedJan 2008 – Jun 2008   Employment Duration6 mos “Determination of the T cell Epitopes for the HIV subtype C virus.” Extensively worked on the key softwares and tools available for the peptide prediction. Worked on the various tissue culture techniques like PBMC separation, ELISPOT assay,Virus cultivation ,DNA isolation etc. “Full length sequencing of HIV genome” Worked on the Molecular biology techniques like PCR, Real Time PCR,Gel Electrophorosis,DNA purification, Extraction of DNA from Gel, Cloning.

Research Interest

 1.Media design and process optimization techniques using DOE /QbD approach.  2.Operation, control and troubleshooting various bioprocess equipments. 3.Analytical techniques (UV-VIS Spectrophotometer, HPLC, ) 4.Handling and operation of various downstream processing techniques (Tangential flow filtration, protein precipitation, Chromatography ). 5.Have sound knowledge and decent experience in GMP and GLP. 6. Application of statistical process control (SPC) strategies. 7. Multi variate data analysis