Nicola Giangregorio

Biography

"WORK EXPERIENCE • Dates (from – to) 19/03/2001 - to date • Name and address of employer CNR (National Council of Research) ""Centre for the study of mitochondria and energetic metabolism"", now become IBBE (Institute of Biomembranes and Bioenergetics), Via Amendola 165 A, 70126 Bari (Italy) • Type of business or sector Life Sciences - Biochemistry and Molecular biology • Occupation or position held CNR Researcher • Main activities and responsibilities Scientific research • Dates (from – to) 01/12/1995 - 18/03/2001 • Name and address of employer Department of Pharmaco-Biology, University of Bari, Via Orabona 4, 70125 Bari (Italy). • Type of business or sector Life Sciences - Biochemistry and Molecular biology • Occupation or position held Graduate laboratory technician • Main activities and responsibilities Scientific research • Dates (from – to) 1995 • Name and address of employer CNR ""Centre for the study of mitochondria and energetic metabolism"", Via Amendola 165/A, 70126 Bari (Italy) • Type of business or sector Life Sciences - Biochemistry and Molecular biology • Occupation or position held Research grant: ""Finalized Project of Biotechnologies and Bio-Instrumentation"" • Main activities and responsibilities Scientific research EDUCATION AND TRAINING • Dates (from – to) 1992-1995 • Name and type of organisation providing education and training Department of Pharmaco-Biology, University of Bari, Via Orabona 4, 70125 Bari (Italy) • Principal subjects/occupational skills covered Cellular Biochemistry and Cellular Pharmacology • Title of qualification awarded PhD in Biochemistry Page 2 - Curriculum vitae of Giangregorio, Nicola • Dates (from – to) 1990 • Name and type of organisation providing education and training University of Bari, (Italy) • Principal subjects/occupational skills covered Biochemistry: "" Purification of the mitochondrial carnitine carrier from rat liver"". • Title of qualification awarded Degree in Pharmacy"

Research Interest

"identification, isolation and purification of membrane proteins; - their reconstitution in artificial membranes (liposomes); - functional and kinetic studies of transport proteins; - identification of primary and secondary structure; - cloning and protein over-expression in bacteria; - site-directed mutagenesis; - genetic pathologies correlated to mutations in mitochondrial carriers; - protein chemical and functional modifications (xenobiotics compounds such as toxic molecules, drugs or newly synthesized compounds); - identification of posttranslational modifications (GSH, H2S, NO, acylations, etc.) and their regulator role of the protein function; - use of bioinformatics for the comparative structural analysis of membrane proteins or docking; - polyclonal antibodies production of membrane proteins and immunochemical detection "