Roberta Squecco

Biography

ROBERTA SQUECCO  Career and career titles : 1993: Degree in Biological Sciences (Voting 110 and Lode / 110 and Encomio). Experimental thesis titled "Biological Characterization Parameters in Bladder Carcinoma: Prognostic Significance in Surface Shapes", conducted at the Department of Clinical Physiopathology, University of Florence. 1994: Exercise of the profession of Biologist (vote at State Exam 150/150) March-October 1994: UILDM (Italian Union of Muscular Dystrophy) Funded Fund financed by TELETHON Project Funds. 1994-1998: Doctoral Program in Physiology (X cycle), four-year doctoral program funded by TELETHON, Italy. Thesis entitled: "Electrophysiological aspects of excitation-contraction coupling in skeletal muscle of amphibious and mammalian normal and dystrophic". Ph.D. degree obtained on 24/02/1999. November 1998-June 1999: TELETHON Study Exchange September 1999: TELETHON Studio Award October 1999 - June 2000: TELETHON Study Exchange 1/07 / 2000-30 / 06/2001: Research Check Holder for the study: "Effects of Ruthenium red, Ryanodina, Caffeine and Sphosphine 1-phosphate on the intracranial load movements recorded by the vaselina double gap technique in single fibers isolated from the skeletal muscle " 1/07 / 2001-30 / 06/2004: Research Check Holder for the Study: "Analysis of the Possible Role of Sphingosine 1-Phosphate as Second Messener in Coupling-Shrinking Process in Skeletal Muscle Cells" 1/07 / 2004- 30/07/2005: co.co.co titular for "Electrophysiological study of the action of sphingosine 1-phosphate on signals and intracellular calcium homeostasis in skeletal muscle cells of the mouse at different stages of differentiation (from mioblasts to myotubes) and fibers differentiated from adult mice " 1/02 / 2006-31 / 08/2006: Owner of co.co.co for the study "Characterization of cardiac and renal cells" 5/07 / 2006- 5/10/2006: co.co.co holder for "Electrophysiological study by intracellular microelettrodes of voltage-dependent ionic channels and activated by mechanical stretching in denervated rat muscle. Evaluation of the atrophic state of chronically denervated muscles performed in individual muscle fibers. " 20/12 / 2006-19 / 12/2007: co.co.co holder for the study "Electrophysiological Characterization of Renal Cells in the Project of TRESOR." 02/04/2007 to 30/04/2007 and 1/01/2008 to 30/04/2008: Occasional collaboration contracts for the following activity: "Study of the effects of Elocalcitol on L channels in human bladder cells" 1/05 / 2008-30 / 04/2009: Contract researcher for the implementation of the research program "Electrophysiological characterization of cardiac, kidney, bone and human fat", (March 17, 2008, Official Gazette No. VII, n3 ). 1/08 / 2009-15 / 09/09: Occasional collaboration agreement for the following activity: Effects of exendin-4 and retinoic acid in neurons. Physiological Ph.D. 28/09 / 2009-28 / 03/2010: Research program on "Isolation of a population of renal stem cells present in urine: phenotypic characterization and analysis of differentiation potential", responsible Prof. Paola Romagnani, Dip. to Clinical Physiopathology 1/07 / 2010-30 / 06/2011: Annual CIMMBA 2010 scholarship entitled: "Electrophysiological and Mismatch Abnormalities Excitement-Myocardial Contractions Predisposing to Arrhythmia and Sudden Heart Disease in Patients with Hypertrophic Cardiomyopathy and in transgenic models of the disease " From 5/11/2012 to today: Full-Time Researcher (Law 240/10) at the Department of Experimental and Clinical Medicine for the Research Activity "Electrophysiological Studies of Trans-Membrane Ion Currents in Voltage-dependent Ion Channels, Exchangers ionic and ionic pumps expressed in stem cells, pluripotent cells and adult cells " 04/04/2017: National Scientific Enabling Competitive Sector: 05 / D1, Band: 2 October 1997- Visiting scientist: at the laboratory led by Prof. Jeanne A. Powell of the Department of Biological Sciences, Smith College (Northampton, MA, USA), where he studied and trained to learn the techniques of Patch clamp , preparation of primary cell cultures of skeletal myoblasts, cell lines.

Research Interest

Skeletal muscular tissue, with particular reference to coupling excitation, differentiation and damage (by eccentric contraction, denervation and aging). The role of bioactive lipids (sphingosine 1-phosphate) on such processes. • Heart muscle tissue with particular reference to differentiation, fibrosis and cardiomyoplasty. • Characterization of the bioelectric properties of stem cells (adipose, renal, cardiac, mesenchymal) with possible implications in cellular therapy. • Neurons and neurodegenerative diseases. • Functionality of the smooth muscle of the gastrointestinal tract: role of hormonal substances (relaxin, orexine, obestatin) in modulating the motility of the gastrointestinal tract. The aforementioned research lines have led to a scientific production that includes over 80 conference communications and 44 extensible publications.