Stefania Brocca
Associate Professor
Department of Biotechnology and Biosciences
University of Milano-Bicocca
Italy
Biography
Stefania Brocca is Researcher at the Department of Biotechnology and Biosciences at the University of Milano-Bicocca. she obtained his degree in Biological Sciences from the University of Milan (1991), discussing an experimental thesis entitled "Study of Mutation G1479-> A1479 in the Coding Gene for the Affected Patient β-Galactosidase from GM1 Infant Gangliosidosis, "carried out at the Department of Biology and Genetics of Microorganisms of the same University. In 1991 he obtained a ministerial scholarship for access to the School of Specialization in Biotechnological Applications. In 1994 he obtained a degree in specialization (cum laude), discussing an experimental thesis entitled "Localization and expression of genes coding for lipid Candida rugosa" at the Department of Physiology and Biochemistry General. From 1996 to 1998 he worked as a post doc in Germany at the Institut für Technische Biochemie of the University of Stuttgart, dedicating himself to the overexpression in Pichia pastoris of lipase of industrial interest. Since November 1999, as an assigner, she has been researching on the topic of lipase protein engineering at the Department of Biotechnology and Biosciences at the University of Milano-Bicocca.
Research Interest
PROTEIN ENGINEERING AND INDUSTRIAL ENZYMOLOGY, PROTEIN STRUCTURE, STABILITY AND AGGREGATION STUDITED BY FT-IR SPECTROSCOPY, PROTEOMICS AND BIOPOLYMER MASS SPECTROMETRY, STRUCTURES, PHYSIOLOGICAL ROLES AND PATHOGENIC POTENTIAL OF AMYLOIDOGENIC PROTEINS.
Publications
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Brocca S, Persson M, Wehtje E, Adlercreutz P, Alberghina L, et al. (2000) Mutants provide evidence of the importance of glycosydic chains in the activation of lipase 1 from Candida rugosa. Protein Science; 9 : 985-990.
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Mileto D, Brocca S, Lotti M, Takagi M, Alquati C, et al. (1998) Characterization of the Candida rugosa lipase system and overexpression of the lip1 isoenzyme in a non-conventional yeast. Chemistry and physics of lipids; 93 : 47-55.
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Fusetti F, Brocca S, Porro D, Lotti M. (1996) Effect of the leader sequence on the expression of recombinant C. rugosa lipase by S. cerevisiae cells. Biotechnology letters; 18 : 281-286.