specialist in microbiology and virology
Elaboration of Research Projects with application of bio-molecular techniques: Techniques for sterilization of culture media, purification of bacterial strains, methods of preservation of bacterial cultures, isolation and identification of the main microbial groups, antibiogram, MIC (assay of the minimum inhibitory concentration), MBEC (assay of the minimum concentration eradicating the biofilm). of genomic prokaryotic DNA and RNA, purification, quantification and separation of nucleic acids on agarose gel. Genomic DNA extraction in agarose plugs, PFGE, enzymatic restriction, Northern and Southern blot, hybridization and simple PCR (Polymerase Chain Reaction) and multiplex, RT-PCR.Techniques for the study of bacterial resistance mechanisms to antibiotics (beta-lactams and glycopeptides) in Staphylococci and Pseudomonas from patients with cystic fibrosis. Study on bacterial biofilm.