Di Resta Chiara

Biography

Expertise areas Electrophysiology, genetics, molecular and cellular biology Techniques acquired Patch-clamp on cell and slice using the whole-cell (in voltage and current clamp) and single channel (in cell-attached and out-side out) Assembly and maintenance of a patch-clamp set-up Use of optical microscope Maintaining both primary cultures (neurons dissociated from rat brain slices) and immortalized cell lines (HEK 293, SY5Y, F11, CHO) Ability to operate in sterility Transient transients of eukaryotic cells using different methods (Ca 2+ -phosphate method , Lipofectamine, Fugene) Dimensitivity in working with mice, rats and cavities Extraction of DNA from blood through both classical methods (phenol chloroform) and automatic extractor Extraction of RNA from human lymphocytes PCR Reverse transcription Establishing protocols for gene sequencing MLPA (multiplex ligation-dependent probe amplification) Sequencing using an automatic sequencer (ABI 3730, Applied Biosystem) DHPLC Next generation sequencing Protein extraction from cultured cells Western-blot Bacterial cultures (amplification and plasmid DNA extraction, electrocompatible E. coli strains, electrotransformation of E. coli strains) Cloning of genes in expression vectors Mutagenesis Course of study November 2015 Researcher in Clinical Pathology (Med / 05), San Raffaele Health and Life University June 2011-today School of Specialties in Clinical Pathology , University of Pavia November 2008-today Post-doctoral fellowship at the Genomics Unit for the Diagnosis of Human Pathologies, Department of Genetics and Cell Biology, San Raffaele Hospital, Milan November 2008 PhD in Molecular and Translacial Medicine at the Cell Physiology Laboratory of the Department of Biotechnology and Biosciences, University of Milan-Bicocca Final Voting: Excellent January 2007 Enabling Biologist profession and enrolling in the albo October 2005 Five Year Degree in Biological Sciences at the Cell Physiology Laboratory of the Department of Biotechnology and Biosciences, University of Milan-Bicocca University. Final vote: 108/110 July 2000 Classical Matura at the "E.Majorana" Classical Lyceum, Desio (Mi) Professional experiences November 2008 - October 2015: project collaboration at the Genomics Unit for the Diagnosis of Human Pathologies, Department of Genetics and Cell Biology, San Raffaele Hospital, Milan October 2008 - October 2013 : Professorships contracted from 01/10 to 23/10 for the tutoring of the didactic laboratory of Experimental Biology - (physiology module) - within the course of the three-year degree in Biological Sciences, University of Milan-Bicocca. November 2005 - November 2008: Ph.D. in Molecular and Translational Medicine at the Cellular Physiology Laboratory of the Central Nervous System of the Department of Biotechnology and Biosciences of the Milan-Bicocca University. September 2004 - October 2005: thesis at the Cellular Physiology Laboratory of the Central Nervous System of the Department of Biotechnology and Biosciences of the Milan-Bicocca University. Other skills and attitudes Foreign languages : English written: great spoken: good understanding: good Computer knowledge : Mac OS X, Windows XP, Office Pack (Excel, Word, Power Point), Origin, Axon Laboratory, pClamp, Internet Explorer, Coreldraw, Acrobat Reader, Sequencher, Oligo 7, Gene Mapper 7 Awards and distinction September 2013 : First best poster award for the project entitled " What we do not know about the genetic basis of Brugada Syndrome" May 2013 : Appointed as "full member young scientist" of the EFLM (European Federation of Clinical Chemistry and Laboratory Medicine) Working Group on personalized laboratory medicine personalized laboratory medicine June 2008 - September 2008 : scholarship from the "LaborLab" project by Regione Lombardia January 2007 - November 2007 : scholarship from "Global Grant INGENIO-LOMBARDIA", Lombardy Region October 2005 : Telethon Award for studies on neuronal nicotine neuronal receptor alfa2 subunit associated with neuronal epilepsy

Research Interest

Electrophysiology, genetics, molecular and cellular biology