Global

Gastroenterology Experts

Nataliia Pavliuchenko

Senior DSP Technician
Technical
Benta Pharma Industries Lebanon
Lebanon

Biography

The main field is developing and providing test systems (ELISA-kits). My responsibilities include: • Bacterial lysis (lysozyme, DNAse, sonication, centrifugation). • Affinity purification of recombinant proteins: GST-fusion system, Ni-agarose, Ni-toyopearl, profinity eXact. • SDS-electrophoresis (Coomasie staining, Silver Staining). • Western Blot, Wet/Semi-dry (Membranes: PVDF, nitrocellulose; Staining of the membranes: Amido Black, Ponceau S red; Substrates for HRP: ECL, Stabilized TMB). • Purification of IgG using resin with immobilized protein A,G. • Purification of IgY from yolk. • ELISA (direct,indirect,sandwich), from coating of the Ag to reading of the results. • Preparing of the reports. • Supervising and training of new members of the department. Also during the work using such methods: • RT-PCR, PCR. • DNA-electrophoresis. • FITC conjugation with antibodies. • Work with animals (immunization, control of immune answer on antigen). Experience Senior DSP Technician at Benta Pharma Industries- BPI November 2015 - Present Downstream Process Technician at Benta Pharma Industries- BPI January 2014 - November 2015 (1 year 11 months) Laboratory Technician September 2009 - February 2014 (4 years 6 months) Education Institute of Biology Master's degree, Immunology, Microbiology and Virology, 2005 - 2011 Holosiivsky Lyceum #241  

Research Interest

 Biology

Global Experts from Lebanon

Global Experts in Subject

Share This Profile
Recent Expert Updates
  • Matthew L Stone
    Matthew L Stone
    pediatrics
    University of Virginia Health System; Charlottesville, VA
    United States of America
  • Dr.   Matthew
    Dr. Matthew
    pediatrics
    University of Virginia Health System; Charlottesville, VA
    United States of America
  • Dr.  L Stone Matthew
    Dr. L Stone Matthew
    pediatrics
    University of Virginia Health System; Charlottesville, VA
    United States of America
  • Dr.  L Stone
    Dr. L Stone
    pediatrics
    University of Virginia Health System; Charlottesville, VA
    United States of America
  • Dr. Matthew L Stone
    Dr. Matthew L Stone
    pediatrics
    University of Virginia Health System; Charlottesville, VA
    United States of America
  • Dr.  R Sameh
    Dr. R Sameh
    pediatrics
    King Abdul Aziz University
    United Arab Emirates
  • Dr.   R Ismail,
    Dr. R Ismail,
    pediatrics
    King Abdul Aziz University
    United Arab Emirates
  • Sameh R Ismail,
    Sameh R Ismail,
    pediatrics
    King Abdul Aziz University
    United Arab Emirates
  • Dr.   Sameh R Ismail,
    Dr. Sameh R Ismail,
    pediatrics
    King Abdul Aziz University
    United Arab Emirates
  • Dr.   William
    Dr. William
    pediatrics
    Maimonides Medical Center
    United States of America