Celine Jeanty

Biography

Celine Jeanty Research Laboratory Technician Luxembourg Institute of Health In vivo experimentation:  > General methods for Zebrafish care, breeding and raising (Zebtech) > Microinjection into zebrafish embryos > Whole mount RNA in situ hybridization on zebrafish embryos Cellular biology:  > Cytotoxicity assays on cardiac cell lines Molecular biology:  > RNA extraction from cardiac cell lines and zebrafish heart ventricles > Gene expression quantification following MIQE guidelines (primers design &validation, quantification BioRad & Applied) > µarray (GEO/NCBI data submission) Computeur skills:  > Software: Microsoft office, Excel (advanced), Beacon designer (primer design), BioEdit (sequence alignment), qBase+ (gene expression analysis), GraphPad Prism (graph&statistics) > Bioinformatics: Molecular biology databases and online tools > Bibliography: Endnote, Mendeley, PubCrawler Norlux Systeme Biomedicine  https://www.lih.lu/page/departments/lrno-ns-norlux-systems-biomedicine-1364 > Cytotoxicity assays on adherent and spheroid cell lines > EC50 determination > Mycoplasma detection and prevention Publications: Analysis of the dynamic co-expression network of heart regeneration in the zebrafish. Rodius S, Androsova G, Götz L, Liechti R, Crespo I, Merz S, Nazarov PV, de Klein N, Jeanty C, González-Rosa JM, Muller A, Bernardin F, Niclou SP, Vallar L, Mercader N, Ibberson M, Xenarios I, Azuaje F. Sci Rep. 2016 May 31;6:26822. PMID:27241320 Transcriptional response to cardiac injury in the zebrafish: systematic identification of genes with highly concordant activity across in vivo models. Rodius S, Nazarov PV, Nepomuceno-Chamorro IA, Jeanty C, González-Rosa JM, Ibberson M, da Costa RM, Xenarios I, Mercader N, Azuaje F. BMC Genomics. 2014 Oct 3;15:85. PMID:25280539 Publications: Analysis of a gene co-expression network establishes robust association between Col5a2 and ischemic heart disease Azuaje F, Zhang L, Jeanty C, Phul SL, Rodius S, Wagner D. BMC Med Genomics. 2013 Apr 10;6:13. PMID:23574622 An optimized protocol for microarray validation by quantitative PCR using amplified amino allyl labeled RNA. Jeanty C, Longrois D, Mertes PM, Wagner DR, Devaux Y. BMC Genomics. 2010 Oct 7;11:542. PMID: 20929564  Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation. Rouy D, Ernens I, Jeanty C, Wagner DR. Anal Biochem. 2005 Mar 15;338(2):294-8. PMID: 15745750     Universite de Lorraine Luxembourg

Research Interest

Research orphan program