M Kamran Azim, Humera Inayat
Cell culture
University of Karachi
Pakistan
Biography
Manipulation of cell culture media by elicitors is one of most important strategies of biotechnology to induce secondary metabolism for the production of valuable metabolites. Natural elicitors such as chitosan are exogenous biotic compounds extracted from arthropods exoskeleton and fungal cell walls often act as specific elicitors in a number of plant cell cultures for efficient induction of valuable medicinally secondary metabolites. In this investigation, inducing effect of chitosan on physiological, biochemical and molecular parameters were investigated in cell suspension cultures of Scrophularia striata Boiss. Cells were treated with 10 mg/L chitosan and harvested for 3, 5 and 7 days after elicitation. Luteolin (Lut) and Myricetin (Myr) quantified by highperformance liquid chromatography (HPLC) Manipulation of cell culture media by elicitors is one of most important strategies of biotechnology to induce secondary metabolism for the production of valuable metabolites. Natural elicitors such as chitosan are exogenous biotic compounds extracted from arthropods exoskeleton and fungal cell walls often act as specific elicitors in a number of plant cell cultures for efficient induction of valuable medicinally secondary metabolites. In this investigation, inducing effect of chitosan on physiological, biochemical and molecular parameters were investigated in cell suspension cultures of Scrophularia striata Boiss. Cells were treated with 10 mg/L chitosan and harvested for 3, 5 and 7 days after elicitation. Luteolin (Lut) and Myricetin (Myr) quantified by highperformance liquid chromatography (HPLC)
Research Interest
Cell culture
