Dr Chloe Bennati-granier

Biography

Dr Chloe Bennati-Granier is a Post-Doctoral Research scientist in the Diet and Glycobiome Group which is interested in the interaction between bacteria and mucus in the human gastrointestinal tract. Dr Chloe Bennati-Granier started to work with Carbohydrate-Active Enzymes (CAZymes) during my Master project, and more especially on the oxidative enzymes active on lignin. She studied the ability of the bacteria Streptomyces for the degradation of micropollutants of wastewater by focusing on laccases enzymes. This work was part of a global project which aims to develop a complementary treatment using constructed wetlands systems and to promote growth of microorganisms able to remove the micropollutants. During her PhD, she conducted research on studying new fungal CAZymes, the lytic polysaccharide monooxygenases (LPMOs) in the context of second generation bioethanol. The first part of the work was to identify new fungal LPMOs and to express them by using the heterologous system Pichia pastoris. The enzymes were produce small scale and in bioreactor (large scale). The second part was the biochemical characterization of those enzymes. She adapted in vitro enzyme assays, prepared cellulosic substrates, quantified and analysed soluble carbohydrate products by ionic chromatography (DIONEX) and mass spectrometry.

Research Interest

Dr Chloe Bennati-Granier is currently interested in the proteins the bacteria produces to interact with mucins (heavily glycosylated proteins). Her research focuses on the study of CAZymes and the use of those CAZymes in the field of Glycoscience to contribute to new biotechnology applications.