Biochemistry and Molecular Biology
Johns Hopkins Bloomberg School of Public Health
United States of America
Education: PhD, University of Sheffield, 2002 BSc, University of Sheffield, 1998 Overview: Research in the Bailey laboratory focuses on the molecular basis of the processes that relate to protein nucleic acid interaction. The strategy that we use is based primarily on structural studies using X-ray crystallography. A central premise of our work is that in order for structural studies to provide understanding of these processes we must know the structure of the entire assembly that executes the process, captured at each step in the process. From such studies we derive mechanistic models relating the physical features and chemistry of proteins and nucleic acids to their function. Interrogation of these models using mutagenesis, biochemistry and cell-based techniques further relates structure to function and provides a more complete molecular description of the process at hand. Honors and Awards: Johns Hopkins Presidents Frontier Award - Winner (February 2016) Johns Hopkins Presidents Frontier Award - Finalist (January 2015) Shikani/El Hibri Prize for Discovery and Innovation - JHSPH (February 2015) Ho-Ching Yang Memorial Faculty Award - JHSPH (May 2010)
CRISPR-Cas, Structural Biology, Protein Nucleic Acid Interaction
Chen H, Choi J, Bailey S (2014) Independent Cut Site Selection by the Two Nuclease Domains of the Cas9 RNA-guided Endoribonuclease. J. Biol. Chem. 289: 13284-13294.
Mulepati S, Heroux A, Bailey S (2014) Crystal structure of a CRISPR RNA-guided surveillance complex bound to a ssDNA target. Science 345: 1479-1484.
Estrella MA, Kuo FT, Bailey S (2016) RNA activated DNA cleavage by the Type III-B CRISPR-Cas effector complex. Genes & Dev. 30(4): 460-470.