Michigan Technological University
United States of America
Dr. Tiwari is a broadly trained protein chemist and cell biologist. His research is in the area of ‘protein aggregation diseases’ with special emphasis on age related neurodegenerative disorders such as Amyotrophic Lateral Sclerosis (ALS), Alzheimer's disease (AD), Parkinson's disease (PD), prion diseases, and Huntington’s disease (HD). In these diseases, misfolded proteins are observed as intracellular or extracellular aggregates at the end stage of the disease. However, the underlying mechanism(s) by which these aggregated proteins impair cellular function(s) and cause toxicity is not clear and is a subject of intense debate. Tiwari laboratory is applying unique biochemical and biophysical approaches (including novel fluorescent molecules) to characterize the inherent vulnerability and instability of proteins associated with different neurodegenerative diseases. The long-term research goal of Tiwari lab is to understand consequences of protein misfolding and aggregation in vitro and how it relates to misfolding and aggregation in vivo, and its implication for neurodegenerative diseases.
Protein misfolding and aggregation diseases Consequences of protein misfolding in vitro and how it relates to misfolding in vivo Molecular crowding
Dorh N, Zhu S, Dhungana KB, Pati R, Luo FT, Liu H, Tiwari A*. BODIPY-Based Fluorescent Probes for Sensing Protein Surface-Hydrophobicity. Sci Rep. (2015) 5:18337.
Zhang J, Yang M, Mazi W, Adhikari K, Fang M, Xie F, Valenzano L, Tiwari A*, Luo F-T, and Liu H. Unusual Fluorescent Responses of Morpholine-functionalized Fluorescent Probes to pH via Manipulation of BODIPY’s HOMO and LUMO Energy Orbitals for Intracellular pH Detection. ACS Sensors (2016) 1(2):158-165.
Zhang J, Li C, Dutta C, Fang M, Zhang S, Tiwari A*, Werner T, Luo F-T, Liu H. A novel near-infrared fluorescent probe for sensitive detection of β-galactosidase in living cells. Analytica Chimica Acta (2017) 968:97-104.