Michigan Technological University
United States of America
It is well-established that in animal cells phosphoinositides play a central role in signal transduction by undergoing signal-stimulated hydrolysis to generate second messengers. However, the biochemical aspects of phosphoinositides in plant cells is not well-understood. Dr. Murthy's research group is investigating the structure, metabolism and biological role of phosphoinositides in plant cells. In their effort to characterize the structure of phosphoinositides in barley seeds, they discovered the presence of novel scyllo-inositol containing phosphoinositides. Currently, they are investigating the biosynthesis, subcellular localization, and biological role of this novel class of phosphoinositides. In addition, the involvement of phosphoinositides in gibberellin-triggered alpha-amylase synthesis is being investigated. A variety of purification techniques such as chromatography, high-voltage paper electrophoresis, HLPC, and spectroscopic techniques such as NMR, 2D NMR, IR, and GC-MS is employed in this project. The second messenger role of inositol-1,4,5-triphosphate has generated a great deal of interest in the structure and metabolism of inositol phosphates. Phytic acid, myo-inositol hexakis phosphate, is the major storage form of inositol phosphates. Recent work has established the presence of phytic acid in a variety of micro-organisms and animal cells. Phytases are the primary enzymes responsible for the degradation of phytic acid. Dr. Murthy's research group is investigating various catalytic and structural aspects of phytases including; specificity of hydrolysis, molecular weight and subunit structure, amino acid sequence and molecular biology.
Structure, metabolism, and biological role of phosphoinositides in plant cells, Catalytic and structural aspects of phytases, including specificity of hydrolysis, molecular weight and subunit structure, amino acid sequence, and molecular biology, Biosynthesis, subcellular localization, and biological role of novel scyllo-inositol containing phosphoinositides
M. Yang, S. Johnson, P.P.N. Murthy (2012) Enhancement of alkaline phytase production in Pichia pastoris: influence of gene dosage, sequence optimization and expression temperature. Protein Express. Purif. 84, 247-254.
P.P.N.Murthy, M. Thompson, K. Hungwe (2014) The Development of a Semester-long, Inquiry-Based Laboratory Course in Upper-Level Biochemistry and Molecular Biology. J. Chemical Education 91, 1909-1917.
Mimi Yang, Sasha Teymorian, Philip Olivares, Pushpalatha P.N.Murthy (2015) Extracellular expression of alkaline phytase in Pichia pastoris: the GAP promoter is more efficient than the AOX1 promoter. Biotechnology Reports 6, 112-118.