Kurt W. Kohn

Biography

Dr. Kohn received an A.B. degree from Harvard in 1952 with majors in Chemistry and Physics, an M.D. degree from Columbia College of Physicians and Surgeons in 1956, and a Ph.D. in Biochemistry and Molecular Biology from Harvard in 1965. After internship at Mount Sinai Hospital in New York, Kohn came to the National Cancer Institute and served as Clinical Associate in the Clinical Pharmacology Service (headed by Paul Condit) 1957-1960. He then spent 2 years as a graduate student and post-doctoral fellow in Paul Doty's laboratory at Harvard, returning to the NCI in 1962 as a member of the Laboratory of Chemical Pharmacology headed by David P. Rall (this Laboratory was a transformation of the earlier Clinical Pharmacology Service). In 1968, Kohn founded the Laboratory of Molecular Pharmacology (LMP) and served as its Chief until 1997. During this time (1961-1997), Kohn's major area of investigation concerned the mechanisms of action of DNA-targeted anticancer drugs. He demonstrated that bifunctional alkylating agents produce DNA interstrand crosslinks and that this is their major cytotoxic action. He elucidated a new mechanism of drug action on DNA, based on the anthramycin group of antibiotics. In 1974, he discovered the DNA filter elution phenomenon in which DNA molecules pass through micropore filters at a rate dependent on DNA strand length. Based on these observations, he developed methodology to measure several types of DNA damage in mammalian cells. This methodology was widely used for more than 20 years in many laboratories to study DNA damage and repair in mammalian cells. By DNA filter elution studies, Kohn and his colleagues in 1979-1982 showed that DNA topoisomerases are targets of action of several clinical anticancer drugs. This led to worldwide interest in topoisomerase-targeted drugs that has continued to the present time. In the 1990's, Kohn and his colleagues began to apply the emerging knowledge of cell cycle checkpoints to study the responses of cancer cells to DNA damage. As an aid to this end, Kohn developed a notation for molecular interaction maps that has recently received considerable interest. Since 1997, Kohn has continued as a Principle Investigator in the LMP, focusing on molecular interaction mapping and computer simulation of bioregulatory networks relevant to cancer and therapeutics. In addition, he continues to collaborate and consult on various LMP projects and with other laboratories. 

Research Interest

Computational Biology