Assistant Professor of Biochemistry
Chemistry & Biochemistry
University of the Sciences
United States of America
I am interested in the study of the mechanisms of macromolecular interactions, including protein-lipid and protein-inhibitor interactions. Efforts are focused on the characterization of proteins using biochemical and spectrometric techniques and the development of kinetic and binding assays for therapeutically important enzymes. One example of a system we are interested in studying is the class of cysteine proteases, which are the proteolytic enzymes that utilize a cysteine residue for their catalytic activity. Especially mammalian papain-like cysteine proteases (also known as thiol-dependent cathepsins), which are medically important since these proteases are essential in osteoporosis, arthritis, immune-related diseases, atherosclerosis, and cancer, as well as in a wide variety of parasitic infections. Targeting this enzyme is therefore very effective in the development of new drugs for a number of diseases. My interest is in developing an assay system for the inhibition of papain-like cysteine proteases as a model system in order to utilize it for the study of enzyme kinetics.
Mechanisms of protein-lipid and protein-inhibitor interactions. Characterizing proteins using biochemical and spectrometric techniques and developing kinetic and binding assays for therapeutically important enzymes
Ghosh AK, Krishnan K, Walters DE, Cho W, Cho H, Koo Y, Trevino J, Holland L, Buthod J. Structure based design: Novel spirocyclic ethers as nonpeptidal P 2-ligands for HIV protease inhibitors. Bioorganic & medicinal chemistry letters. 1998 Apr 21;8(8):979-82.
Nandi D, Lightcap ES, Koo YK, Lu X, Quancard J, Silverman RB. Purification and inactivation of 3-hydroxyanthranilic acid 3, 4-dioxygenase from beef liver. The international journal of biochemistry & cell biology. 2003 Jul 31;35(7):1085-97.
Li Y, Chen F, Putt M, Koo YK, Madaio M, Cambier JC, Cohen PL, Eisenberg RA. B cell depletion with anti-CD79 mAbs ameliorates autoimmune disease in MRL/lpr mice. The Journal of Immunology. 2008 Sep 1;181(5):2961-72.