Prachi N Ghule
Department of Biochemistry
University of Vermont
United States of America
Biography
Prof/Dr Prachi N Ghule has received his PhD inin Applied Biology – University of Mumbai, Mumbai, India2005-2011. Currently, he/she is working as Professor in university of vermont.
Research Interest
Role of Histones and Histone gene regulators during Cell Cycle We are interested in understanding regulation of Histone genes during cell cycle of pluripotent cells (hES cells) and normal or cancer cells. We have identified sub-nuclear domains knows as Histone Locus bodies (HLBs) that are associated with Histone gene clusters and are temporally and spatially organized during G1/S transition of the cell cycle. We are also investigating role of histone (H4) gene regulatory protein (HiNF-P) in vivo, to evaluate the consequence of HiNF-P ablation (complete Knock-out or conditional loss of HiNF-P) in vivo. We know that complete loss of HiNF-P causes embryonic lethality in mice at blastocyst stage. Therefore, we have developed a conditional knock out model, which will help us investigate importance of tissue specific loss of HiNF-P
Publications
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Becker KA, Ghule PN, Lian JB, Stein JL, Van Wijnen AJ, Stein GS. Cyclin D2 and the CDK substrate p220NPAT are required for selfâ€renewal of human embryonic stem cells. Journal of cellular physiology. 2010 Feb 1;222(2):456-64.
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Ghule PN, Dominski Z, Yang XC, Marzluff WF, Becker KA, Harper JW, Lian JB, Stein JL, Van Wijnen AJ, Stein GS. Staged assembly of histone gene expression machinery at subnuclear foci in the abbreviated cell cycle of human embryonic stem cells. Proceedings of the National Academy of Sciences. 2008 Nov 4;105(44):16964-9.
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Filion TM, Qiao M, Ghule PN, Mandeville M, Van Wijnen AJ, Stein JL, Lian JB, Altieri DC, Stein GS. Survival responses of human embryonic stem cells to DNA damage. Journal of cellular physiology. 2009 Sep 1;220(3):586-92.
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Becker KA, Ghule PN, Therrien JA, Lian JB, Stein JL, Van Wijnen AJ, Stein GS. Selfâ€renewal of human embryonic stem cells is supported by a shortened G1 cell cycle phase. Journal of cellular physiology. 2006 Dec 1;209(3):883-93.
