Andrey A. Bobko
Research Assistant Professor
West Virginia University Health Science Center
United States of America
Works as Research Assistant Professor in Biochemistry.
My research interests are the magnetic resonance studies of microenvironment parameters, including acidity (pH), redox, glutathione (GSH), inorganic phosphate (Pi), glucose, oxygenation (pO2). Our lab use Electron Paramagnetic and Nuclear Paramagnetic Resonance (EPR and NMR) Spectroscopy and Imaging techniques and their combination for functional assessment of biological system on cellular, tissues and organ/whole body levels. The development of unique multifunctional paramagnetic probes are the main focus of our lab research, it includes synthesis and characterization of triarylmethyl- and nitroxide-based radicals, manufacture of paramagnetic probes and their nanoassemblies with specific properties (e. g. intracellular or extracellular compartments localization, targeting to specific tissue/organ), implementation of developed probes for functional parameters monitoring. The long term goal of our lab is to develop small animal based clinically-relevant approaches for drug screening and cancer studies using paramagnetic probes and magnetic resonance techniques.
Roshchupkina, GI; Bobko, AA; Bratasz, A; Reznikov, VA; Kuppusamy, P; Khramtsov, VV. "In vivo EPR measurement of glutathione in tumor-bearing mice using improved disulfide biradical probe". Free Radical Biology & Medicine 2008, 45(3), 312-320.
Dhimitruka I, Bobko AA, Eubank TD, Komarov DA and Khramtsov VV. 2013. "Phosphonated trityl probes for concurrent in vivo tissue oxygen and pH monitoring using electron paramagnetic resonance-based techniques", J. Am. Chem. Soc. 135, 5904−5910.
Bobko AA, Dhimitruka I, Zweier JL, Khramstov VV. 2014, "Fourier Transform EPR of Trityl Radicals for Multifunctional Assessment of Chemical Microenvironment", Angew.Chem.Int.Edit. 53, 2735-2738.