COLLEGE OF NURSING & HEALTH
Wright State University
United States of America
Professor, Pharmacology & Toxicology , works in 207 Health Sciences, 3640 Colonel Glenn Hwy, Dayton, OH 45435-0001
Ion Transport and Vascular Cells in Health and Disease, funded by NIH, American Heart Association, and private funding Research involves the role of ion transport abnormalities in the etiology and treatment of diseases (essential hypertension, sickle cell anemia, Alzheimer's disease), and on aging. The main focus of the research is on the characterization, regulation, including signal transduction, and expression of ion transport systems and their relationship to abnormal cellular function and to cellular aging. Main cell models are cultured vascular endothelial cells, smooth muscle cells, red blood cells, lens epithelial cells, and transfected HEK-293 cells. The main techniques used are measurement of ion fluxes by atomic and electrothermal atomic absorption spectrophotometry or by radiotracers, flow cytometry, use of fluorescent dyes, and molecular biological techniques for isolation, cloning, and expression, in Xenopus oocytes and by cell transfection, of transport systems, in particular, the K-Cl cotransporter.
Canonical Bcl-2 motifs of the Na+/K+ pump revealed by the BH3 mimetic chelerythrine: early signal transducers of apoptosis? Lauf PK, Heiny J, Meller J, Lepera MA, Koikov L, Alter GM, Brown TL, Adragna NC, Cell Physiol Biochem. 2013;31(2-3):257-76. doi: 10.1159/000343366. Epub 2013 Feb 22. Erratum in: Cell Physiol Biochem. 2013;31(4-5):744, PMID: 23466888
Interaction between Na-K-ATPase and Bcl-2 proteins BclXL and Bak, Lauf PK, Alqahtani T, Flues K, Meller J, Adragna NC., Am J Physiol Cell Physiol. 2015 Jan 1;308(1): C51-60. doi: 10.1152/ajpcell.00287.2014. Epub 2014 Oct 15, PMID: 25318106
K-Cl cotransporters, cell volume homeostasis, and neurological disease, Kahle KT, Khanna AR, Alper SL, Adragna NC, Lauf PK, Sun D, Delpire E., Trends Mol Med. 2015 Aug;21(8):513-23. doi: 10.1016/j.molmed.2015.05.008. Epub 2015 Jul 1. Review., PMID: 26142773